Přístroje

The laboratory is classified for handling of the open sources of ionizing radiation in quantities authorized for laboratories of II category according to Czech by law 422/2016 Sb for R&D and educational purposes. It is currently authorized to work with the main radioactive isotopes used in research, e.g. ³H, ¹⁴C, ³²P, ³³P, ³⁵S, ⁵¹Cr, ⁵⁴Mn, ⁵⁵Fe, ⁹⁹Tc, and ¹²⁵I.

The key instrument of the laboratory is a glove box with tritiation manifold (RC-TRITEC AG) suitable for handling 100 to 1000 Ci of carrier-free tritium gas. Instruments for characterization of radioactively labeled compounds: ³H NMR BRUKER Avance II 300 MHz. Liquid scintillation analyzer TriCarb 2900TR (Perkin Elmer) for activity assays of small amounts of α, β, and γ emitters. The Gamma Counter Wizard 1470 and high throughput WIZARD² 2470 (Perkin Elmer). Radio-TLC scanner RITA (RAYTEST). Analytical-preparative radio-HPLC Waters 600 with radio-detector Ramona. Analytical-semipreparative radio-HPLC Waters Alliance e2695 system with radio-detector Ramona suitable also for radio-metabolite detection. MicroBeta² plate counter - high throughput scintillation detection (LSC, SPA, FlashPlates, Filtermat...) of variety of radionuclides (such as ³H, ¹⁴C, ³²P and ¹²⁵I-labeled compounds, etc) directly in 96/384-well plates. Analytical radio-LC-MS Waters Alliance e2695 system with SQ detector 2 furnished with ESI, APCI probe and radio-detector Ramona. GC-MS Agilent 6890N gas chromatograph with 5975B quadrupole mass spectrometer.

For detailed description please visit list of instruments.