The laboratory is classified for handling of the open sources of ionizing radiation in quantities authorized for laboratories of II category according to Czech by law 422/2016 Sb for R&D and educational purposes. It is currently authorized to work with the main radioactive isotopes used in research, e.g. 3H, 14C, 32P, 33P, 35S, 51Cr, 54Mn, 55Fe, 99Tc, and 125I.
The key instrument of the laboratory is a glove box with
tritiation manifold (RC-TRITEC AG) suitable for handling 100 to 1000 Ci of carrier-free tritium gas. Instruments for characterization of radioactively labeled compounds:
3H NMR
BRUKER Avance II 300 MHz. Liquid scintillation analyzer
TriCarb 2900TR (Perkin Elmer) for activity assays of small amounts of α, β, and γ emitters. The
Gamma Counter Wizard 1470 (Perkin Elmer) and high throughput WIZARD
2 2470 (Perkin Elmer).
Radio-TLC scanner RITA (RAYTEST). Analytical-preparative
radio-HPLC Waters 600 with radio-detector Ramona. Analytical-semipreparative
radio-HPLC Waters Alliance e2695 system with radio-detector Ramona suitable also for radio-metabolite detection. MicroBeta
2 plate counter - high throughput scintillation detection (LSC, SPA, FlashPlates, Filtermat,...) of variety of radionuclides (such as
3H,
14C,
32P and
125I-labeled compounds, etc) directly in 96/384-well plates. Analytical
radio-LC-MS Waters Alliance e2695 system with SQ detector 2 furnished with ESI, APCI probe and radio-detector Ramona.
GC-MS Agilent 6890N gas chromatograph with 5975B quadrupole mass spectrometer.
For detailed description please visit list of instruments.
