The laboratory is classified for handling of the open sources of ionizing radiation in quantities authorized for laboratories of II category according to Czech by law 422/2016 Sb for R&D and educational purposes. It is currently authorized to work with the main radioactive isotopes used in research, e.g. 3H, 14C, 32P, 33P, 35S, 51Cr, 54Mn, 55Fe, 99Tc, and 125I.

The key instrument of the laboratory is a glove box with tritiation manifold (RC-TRITEC AG) suitable for handling 100 to 1000 Ci of carrier-free tritium gas. Instruments for characterization of radioactively labeled compounds: 3H NMR BRUKER Avance II 300 MHz. Liquid scintillation analyzer TriCarb 2900TR (Perkin Elmer) for activity assays of small amounts of α, β, and γ emitters. The Gamma Counter Wizard 1470 (Perkin Elmer). Radio-TLC scanner RITA (RAYTEST). Analytical-preparative radio-HPLC Waters 600 with radio-detector Ramona.